Document Details

Document Type : Project 
Document Title :
DEVELOPMENT OF REAL-TIME PCR FOR THE DETECTION OF MALARIA IN SAUDI
: دراسة تشخيص مرض الملاريا بواسطة البلمرة التسلسلية
 
Subject : MEDICAL PARASITOLOGY 
Document Language : Arabic 
Abstract : Malaria transmission occurs in Saudi Arabia and especially endemic in the lowlands of the Asir region in the southwestern province. Imported cases have been reported. Sensitive routine laboratory techniques for rapid and accurate malaria diagnosis are therefore desirable to facilitate the identification of individuals infected with the malarial parasites and to follow up the progress of treatment of such cases with appropriate drugs. Traditional diagnosis, based on the microscopic examination of Giemsa-stained thick and thin films remains the main standard for diagnosis. Although it has good sensitivity and allows species identification and parasite counts, it is time consuming, requires microscopical expertise and maintenance of equipment. Molecular diagnostic techniques based on the detection of nucleic acid (including PCR; Real time-PCR) are now highly considered. New studies are desperately needed to reassess the diagnostic techniques used for malaria in the Kingdom. Real time-PCR—a new methodology that employs fluorescent labels to enable the continuous monitoring of amplicon (PCR product) formation throughout the reaction—has recently been adapted to detect all four human malaria parasites indiscriminately and screen large numbers of samples. In this study a total of forty four samples, using whole-blood as well as dried blood, where examined and evaluated by conventional microscopy as well as PCR technologies. Both PCR and Real-time PCR detected showed a higher sensitivity than that of microscopy. Parasites were detected in 29 samples, compared to 26 out of 44 samples were positive with thin blood smear. The real-time PCR assay offers a practical and positive alternative for rapid and accurate diagnosis of malaria infection. The Application of such techniques will be significantly valuable as a diagnostic tool in hospitals and bloodbank screening for malaria infection in endemic area. 
Publishing Year : 1424 AH
2004 AD 
Sponsor Name : kau 
Sponsorship Year : 1424 AH
2004 AD 
Added Date : Monday, November 9, 2009 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
منال بكر جمجومJamjoom, Manal BakurInvestigatorDoctorate 

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 24443.doc doc 

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