Document Details

Document Type : Project 
Document Title :
Successful Propagation of Alkhumra (misnamed as Alkhurma) Virus in C6/36 Mosquito Cells
زراعة فيروس الخمرة في خلايا مستنبطة من الزرع النسيجي للبعوض )C6/36(
 
Subject : Successful Propagation of Alkhumra (misnamed as Alkhurma) Virus in C6/36 Mosquito Cells 
Document Language : Arabic 
Abstract : Epidemiological data suggest that Alkhumra (misnamed as Alkhurma) virus (ALKV) is trans-mitted from livestock animals to humans by direct contact with animals or by the mosquito bites, but not by ticks. To assess the ability of the virus to replicate in mosquito cells, serum and plasma of seven acutely febrile patients with clinically suspected ALKV infection reported in Najran, Saudi Arabia in 2009 were inoculated onto Aedes albopictus mosquito cells (C6/36) and directly examined with ALKV-RNA-specific real time RT-PCR as well as indirect immunfluorescence assay (IFA) using ALKV-specific polyclonal antibodies. The iso-lated virus was titrated in the mammalian rhesus monkey kidney cells (LLC-MK2). Five of the seven specimens were RT-PCR- and culture-positive demonstrating cytopathic effects in the form of cell rounding and aggregation appearing on day 3 post inoculation with syn-cytia eventually appearing on day 8 post inoculation. Identification of ALKV-RNA in the cell culture was confirmed with RT-PCR and IFA. The virus titre was 3.2 × 106 tissue culture infective dose 50 (TCID50) per mL. Three more viral passages were successfully made in the C6/36 cells. This is the first description of propagation of ALKV in mosquito cells. 
Publishing Year : 1432 AH
2011 AD 
Number Of Pages : 6 
Sponsor Name : Mohammad Hussain Alamoudi 
Sponsorship Year : 1430 AH
2009 AD 
Added Date : Saturday, April 6, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
طارق أحمد مدنيMadani, Tariq AhmedResearcherDoctoratealkhumra@hotmail.com

Files

File NameTypeDescription
 35392.pdf pdf 

Back To Researches Page