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Document Type |
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Thesis |
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Document Title |
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Overproduction of Bio-cellulose from Acetobacter xylinum by mutation used in the Treatment of Renal Failure زيادة انتاج السليلوز الحيوي من أسيتوباكتر زيلينوم بواسطة الطفرات المستخدم في غشاء غسيل الكلى |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Cellulose is the earth’s major biopolymer and become tremendous economic importance globally. The bacterial cellulose has significant advantages over natural cellulose.Bacterial cellulose (BC), produced by aerobic bacteria received ample of attention due to its unique physiochemical properties compared to plant cellulose. Intense researches on BC mainly focus on biosynthetic process to achieve low-cost preparation and high cellulose production. BC has been used as biomaterial for medical field, electrical instrument and food ingredient. However, BC alone has limited capabilities to fulfil current demand on high-performance biomaterials. Hence, BC composite has been introduced to enhance BC properties through addition of reinforcement materials.Renal replacement therapy is initiated when a substantial or total loss of ability of the human kidneys to remove water, excrete metabolic waste products or maintain body homeostasis occurs. In the strictest sense, the therapy is not one of replacement, merely supplementation of the filtration capacity of the failing kidney. The most commonly used replacement method is haemodialysis, which involves passing the patient’s blood through an artificial kidney or haemodialyser containing a semi-permeable membrane. Blood flows on one side of the membrane, the other side of which is bathed by a dilute electrolyte solution. In chemical engineering terms a haemodialyser may be considered as a simple mass exchanger.Membranes manufactured from synthetic polymers on other hand have much more complex structures in which a thin layer is supported on a coarser substructure. Molecular transport occurs within the thin surface layer, whilst the substructure acts as mechanical support. The substructure may not be uniform but may be subject to structural variations which are consequences of membranes manufactures altering a variety of parameters or using differing polymer blends.Bacterial cellulose is an alternative biomaterial than other cellulose it’s called bio-cellulose. Bio-cellulose has are different on other cellulose producers such as plant. The bacterial cellulose has excellent characteristics and high purity because it is free from other contaminants like waxy aromatic compounds, lignin and hemicelluloses. This kind of cellulose attract scientists for improving of such bacterial strains to attempt for over production because it’s safe in biomedical, food and industry. Bacterial cellulose fibers can be oriented in regular or randomly depending on the type of incubation period for Acetobacter sp. and other bacterial genera synthesize cellulose. Acetobacter xylinum produce sufficient amount of cellulose to warrant commercial interest. Most scientists focus in its production because it produce ultrafine cellulose fibers. In this study our experiments were carried out to compare and screen five isolated and identified Acetobacter xylinum strains for cellulose yields. Some of strains showed the highest productivity, plasmid isolation indicated the Acetobacter xylinum under study contain four different pattern of plasmid DNA molecules. Molecular fingerprinting using RAPD-PCR was done and showed that differences in RAPD patterns produced by our used strains. |
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Supervisor |
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Dr. Salah Eddin Abu Abu |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Added Date |
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Wednesday, August 21, 2019 |
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Researchers
| آلاء محمد السلمى | AlSulami, Alaa Mohammed | Researcher | Master | |
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