Document Details
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Document Type |
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Thesis |
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Document Title |
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Formulation development and evaluation of anticancer activity of Retinoic acid loaded lipid vesicles تطوير صياغة وتقييم النشاط المضاد للسرطان للحويصلات الدهنية المحملة بحمض الريتينويك |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Introduction: Retinoic acid (RA) is a metabolite of vitamin A, mediates the functions of vitamin A1 important for growth and development, and shows pleotropic effects such as anticancer, antimicrobial, antidiabetic activity, to name a few. Multiple research was conducted on RA but mostly on pure drug effect. Till date, Lipid formulation of RA is not evaluated against breast cancer. Very limited work is available on the formulation of RA and its evaluation against different disease.
Aim: Considering previous research, present study was designed for formulation and evaluation of novel RA loaded lipid vesicles for cytotoxicity and anticancer activity.
Methods: Three different concentrations of RA-loaded lipid vesicles formulations F1, F2 and F3 (5, 10, and 25 mg RA) were prepared by “thin film hydration process” by reported standard method. For preparation of formulations, different lipids, cholesterol, and surfactant were used. After formulations preparation and stability testing, it was evaluated for cytotoxic effect and anticancer activity. Formulations were evaluated for morphology, capacity of drug load, Transmission Electron Microscopy (TEM), determination of pH, spreadability and viscosity and in vitro drug release capacity. Its cytotoxic potential was measured by MTT assay.
Results: The particles of formulation was sphericals with nano range in scanning electron microscopy (SEM). pH value of formulation was found to be 6.9±0.14. Spreadability was 56.18±2.65 g cm/sec. Drug loading capacity was 6.69 mg/10 mg of particles. IC50 of F2 and F3 formulation was 0.25 and 0.26 mg/ml while it was 0.55 mg/ml for F1 formulation.
Conclusion: From this study, it can be concluded that F2 lipid formulation of retinoic acid is effective in the treatment of breast cancer. This natural bioactive compound with synthetic medicine can be used to treat cancer and its associated complications more efficiently. Furthermore, these compounds can be used as an adjunct therapy in dose reduction of synthetic medicine.
Keywords: Vitamin A derivative; retinoic acid; formulation development; stability; efficacy; anticancer activity; cell viability assay. |
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Supervisor |
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Dr. Imran Kazmi |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1444 AH
2022 AD |
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Co-Supervisor |
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Prof. Fahad A. Al-Abbasi |
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Added Date |
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Sunday, February 19, 2023 |
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Researchers
| أحمد صالح الغامدي | Alghamdi, Ahmed Saleh | Researcher | Master | |
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